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Directed Evolution : Methods and Protocols, Paperback / softback Book

Directed Evolution : Methods and Protocols Paperback / softback

Edited by Andrew Currin, Neil Swainston

Part of the Methods in Molecular Biology series

Paperback / softback

Description

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle.

Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

Information

  • Format:Paperback / softback
  • Pages:278 pages, 58 Illustrations, color; 5 Illustrations, black and white; XI, 278 p. 63 illus., 58 illus
  • Publisher:Springer-Verlag New York Inc.
  • Publication Date:
  • Category:
  • ISBN:9781071621547
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Information

  • Format:Paperback / softback
  • Pages:278 pages, 58 Illustrations, color; 5 Illustrations, black and white; XI, 278 p. 63 illus., 58 illus
  • Publisher:Springer-Verlag New York Inc.
  • Publication Date:
  • Category:
  • ISBN:9781071621547

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