Fed-Batch Fermentation : A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli Hardback
by G. G. (President and CSO of BioBench LLC, Seattle, USA) Moulton
Part of the Woodhead Publishing Series in Biomedicine series
Hardback
Description
Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production.
It may also be used as a template for the production of recombinant protein product for use in clinical trials.
The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision.
This process can also be done on a small (2L) scale that is scalable to 30L or more.
All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive.
This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.
Information
-
Item not Available
- Format:Hardback
- Pages:188 pages
- Publisher:Elsevier Science & Technology
- Publication Date:01/01/2013
- Category:
- ISBN:9781907568923
Information
-
Item not Available
- Format:Hardback
- Pages:188 pages
- Publisher:Elsevier Science & Technology
- Publication Date:01/01/2013
- Category:
- ISBN:9781907568923
