PCR/RT- PCR in situ : Light and Electron Microscopy Hardback
by Gerard (Universite Claude Bernard, France) Morel, Mireille (Universite Claude Bernard, Villeurbanne Cedex, France) Raccurt
Part of the Methods in Visualization series
Hardback
Description
Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure.
The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene modification, tumor diagnosis, gene therapy, and cellular distribution of rare mRNA copies. PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels.
Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR.
The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation.
They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.
Information
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Out of stock
- Format:Hardback
- Pages:444 pages, 24 Halftones, black and white; 21 Illustrations, color; 237 Illustrations, black and whit
- Publisher:Taylor & Francis Inc
- Publication Date:27/09/2002
- Category:
- ISBN:9780849300417
Information
-
Out of stock
- Format:Hardback
- Pages:444 pages, 24 Halftones, black and white; 21 Illustrations, color; 237 Illustrations, black and whit
- Publisher:Taylor & Francis Inc
- Publication Date:27/09/2002
- Category:
- ISBN:9780849300417
