CRISPR-Cas Methods Hardback
Edited by M. Tofazzal Islam, Pankaj K. Bhowmik, Kutubuddin A. Molla
Part of the Springer Protocols Handbooks series
Hardback
Description
This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing.
Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications.
Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template.
Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.
Information
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Out of Stock - We are unable to provide an estimated availability date for this product
- Format:Hardback
- Pages:278 pages, 58 Illustrations, color; 9 Illustrations, black and white; XIV, 278 p. 67 illus., 58 illu
- Publisher:Springer-Verlag New York Inc.
- Publication Date:30/06/2020
- Category:
- ISBN:9781071606155
Information
-
Out of Stock - We are unable to provide an estimated availability date for this product
- Format:Hardback
- Pages:278 pages, 58 Illustrations, color; 9 Illustrations, black and white; XIV, 278 p. 67 illus., 58 illu
- Publisher:Springer-Verlag New York Inc.
- Publication Date:30/06/2020
- Category:
- ISBN:9781071606155
